In Vitro Culture of Ovine Spermatogonial Stem Cells: Effects of Grape Seed Extracts and Vitamin C

Background: Spermatogonial stem cells (SSCs) transmit genetic information to the next generation. For successful transplantation of these cells, culture medium must be optimized. Objectives: This study investigated natural antioxidant grape seed extract (GSE) in comparison vitamin C SSCs. Methods: A Soxhlet extractor was used prepare ethanolic and acetonic extracts, Clevenger apparatus aqueous extracts seeds. Then, their capacities were determined using DPPH method. The SSCs extracted from lamb testicular tissue by a two-step digestion method, different concentrations GSE for survival, colony formation, expression apoptotic-related genes. Results: results showed that concentration 400 µg/mL about 90% properties based on test; nevertheless, had only 50% at this concentration. significantly decreased viability without any formation when medium. highest survival rate obtained seeds 50 µg/mL, significant difference with (100 1000 µg/mL) (P < 0.05). treated (50 higher 0.05) than control 5 - 25 groups. Conclusions: Aqueous, ethanolic, should not However, use is recommended